The Accuri is our small cell analyser suitable for quantitative cell counting of microbial cultures and plankton samples. It is robust, quick in set up and easy to handle also for non-experts. Sensitivity is sufficient for most marine samples. It has a blue-green laser with four detectors and a red laser with one detector associated.
The rugged small benchtop flow cytometer can be used for shipboard analysis of picoplankton and for sorting radioactively-labeled microorganisms for down-stream analysis. Because of its cuvette-based analysis system, it is a very sensitive system capable of even detecting virus-like particles. The closed mechanical sorter makes it a safe system to work with radioactive material.
This large research flow cytometer is used for high-speed sorting of cellular material for e.g. 16S rRNA cloning and genome sequencing. The machine was upgraded in 2017 and relocated to a guest lab at the Biological Station (BAH) of the Alfred Wegener Institute (AWI) at Helgoland, North Sea. Freshly collected material can be analysed within an hour after sampling and sorted for further processing.
BD Influx Mariner
The Influx is our next-gen flow sorter for multiparameter analysis and high-speed cell sorting for e.g. genome analyses. It is a stream-in-air droplet sorter with a high sensitivity forward scatter detector, four lasers (UV, blue, yellow and red) and four octagon detection units comprising 13 PMTs in total.
Since the machine is built on a robust steel frame on rolls, it is movable and in principle suitable for usage on-board a research ship.
The robotic sampler can be used for sorting cells into a wide variety of collection vials from small PCR tubes to 384 multi-titer plates to microscope slides.
Analysis rates are typically between 1,000 - 10,000 events per second, sort rates depend on the target cell densities and can reach up to 1000 per second. Using optimal settings, the purity is typically >99%.